Pegg, Graham, Sinclair, Billy, Briskey, Leica and Aspden, William (2006) MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia. Scientia Marina, 70 (Supp 2). pp. 7-12. Full text not available from this repository.
Abstract
Planktonic larvae were captured above a shallow coral reef study site on the Great Barrier Reef (GBR) around spring-summer new moon periods (October-February) using light trap or net capture devices. Larvae were identified to the genus or species level by comparison with a phylogenetic tree of tropical marine fish species using mtDNA HVR1 sequence data. Further analysis showed that within-species HVR1 sequence variation was typically 1-3%, whereas between-species variation for the same genus ranged up to 50%, supporting the suitability of HVR1 for species identification. Given the current worldwide interest in DNA barcoding and species identification using an alternative mtDNA gene marker (cox1), we also explored the efficacy of different primer sets for amplification of cox1 in reef fish, and its suitability for species identification. Of those tested, the Fish-F1 and -R1 primer set recently reported by Ward et al. (2005) gave the best results.
| Item Type: | Article |
|---|---|
| Journal / Publication Title: | Scientia Marina |
| Publisher: | Institut de Ciencies del Mar de Barcelona |
| ISSN: | Print ISSN: 0214-8358 Online ISSN: 1886-8134 |
| Related URL(s): | |
| Departments: | Academic Departments > Science, Natural Resources & Outdoor Studies (SNROS) > Forestry and Conservation |
| Depositing User: | Insight Administrator |
| Date Deposited: | 06 Jan 2012 15:07 |
| Last Modified: | 11 Jan 2024 19:30 |
| URI: | https://insight.cumbria.ac.uk/id/eprint/1095 |
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