Genome-wide identification of Fagus sylvatica aquaporins and their comparative spring and summer expression profiles

Israel, David, Durand, Maxime, Salmon, Yann, Zwiazek, Janusz Jerzy and Robson, Matthew (2022) Genome-wide identification of Fagus sylvatica aquaporins and their comparative spring and summer expression profiles. Trees, 37 (3). pp. 683-698.

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Official URL: https://doi.org/10.1007/s00468-022-02376-z

Abstract

European beech (Fagus sylvatica) has been widely studied in terms of its water relations and local adaptation. However, to date, the underlying basis conferring adaptation to differences in water availability are unknown. Therefore, we examined the expression of aquaporins in trees of four different beech provenances representing the southern and northern range margins, as well as core populations, grown in a common garden. We sampled their xylem, phloem and leaf tissue, when leaves had fully expanded, and in late summer. A total of 45 aquaporin isoforms were identified in the beech genome, of which 35 were detected across all sampled tissues. In our phylogenetic analysis, beech aquaporins clustered into the five subfamilies found in other woody species. Members of the plasma membrane intrinsic protein subfamily generally displayed the highest levels of expression, followed by tonoplast intrinsic proteins. Isoforms of the remaining subfamilies, Noduline-26-like intrinsic proteins, small basic intrinsic proteins and uncharacterised intrinsic proteins, were expressed at very low to moderate levels. The expression of most isoforms was stable or declined from spring to summer. Leaves followed a different expression profile from that of vascular tissues, whereas both phloem and xylem were found to express the same Fs MIPs. Tissue-specific aquaporin expression was very similar amongst the four beech provenances, indicating that there is no inherent difference in the capability of these provenances to regulate aquaporin activity. The general decrease in Fs MIP expression toward the end of the growing period indicates that aquaporins are involved in tree water relations and growth.

Item Type: Article
Journal / Publication Title: Trees
Publisher: Springer
ISSN: 1432-2285
Departments: Institute of Science and Environment > Forestry and Conservation
Additional Information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
Depositing User: Anna Lupton
Date Deposited: 21 Dec 2022 11:32
Last Modified: 11 May 2023 15:15
URI: https://insight.cumbria.ac.uk/id/eprint/6767

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