Stains for entomological microtechnique: simple stains for whole mounts and dissection

Chick, A.I.R. (2020) Stains for entomological microtechnique: simple stains for whole mounts and dissection. Zootaxa, 4790 (3). pp. 447-472. Full text not available from this repository.

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Abstract

Slide mounted entomological specimens often require the aid of contrast techniques to improve the clarity of morphological
characteristics. Methods can involve the use of techniques such as Phase contrast, Dark field or differential interference
contrast microscopy (DIC), however where an entomologist may only have access to simple brightfield microscopy
chemical staining of the specimen may be used to improve contrast. For whole mounts of entomological specimens, a
single stain, occasionally two, are often used, in comparison to histological sections that often employ multiple stains
in complex protocols. A number of authors have proposed different stains and staining methods for a number of insect
groups with few considering the long term qualities of the stain, it has previously been shown that aniline dyes are prone
to fading in Canada Balsam mounts, and that some stains fade even when protected from sunlight. This paper aims to
summarise the knowledge of stains used for entomological specimens and provide details on the archival qualities

Item Type: Article
Journal / Publication Title: Zootaxa
Publisher: Magnolia Press
ISSN: 1175-5334
Departments: STEM
Additional Information: Zootaxa does not support archiving Full Text in institutional repositories
Depositing User: Andy Chick
Date Deposited: 15 Jun 2020 12:20
Last Modified: 15 Jun 2020 12:20
URI: http://insight.cumbria.ac.uk/id/eprint/5574

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