A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis

Purdy, Sarah J., Bussell, John D., Nelson, David C., Villadsen, Dorthe and Smith, Steven M. (2010) A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis. Journal of Plant Physiology, 168 (3). pp. 263-269. Full text not available from this repository.

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Official URL: http://dx.doi.org/10.1016/j.jplph.2010.07.001

Abstract

Plants respond to cold by transcriptional and metabolic responses which underlie tolerance and acclimation mechanisms, but details at the molecular level are incomplete. Here we describe KOLD SENSITIV-1 (KOS1), a new gene required for responses to cold. KOS1 protein is predicted to have coiled-coil, Structural Maintenance of Chromosomes and nuclear-targeting domains. GFP-labeled KOS1 localizes to the nucleus. Null mutants could not be isolated but two independent knockdown T-DNA mutants were obtained. Growth and development of kos1 knockdown mutant plants was comparable to wild type when grown at 21 °C. However, when grown at 4 °C these mutants exhibited accelerated leaf yellowing and smaller rosette size than wild type. Quantitative RT-PCR revealed that in the cold kos1 mutants had reduced expression of cold-responsive transcripts COR15A, COR15B, BAM3 and AMY3. Metabolite profiling revealed that ascorbate levels were lower in the mutants in the cold relative to wild type. KOS1 therefore represents a new gene that influences the regulation of transcript and metabolite levels in response to prolonged chilling temperatures.

Item Type: Article
Journal or Publication Title: Journal of Plant Physiology
Publisher: Elsevier
ISSN: 1618-1328
Departments: Faculty of Health and Science > Science, Natural Resources and Outdoor Studies > Forestry, Conservation & Applied Science
Depositing User: Anna Lupton
Date Deposited: 15 Jun 2016 14:14
Last Modified: 13 Jun 2017 11:49
URI: http://insight.cumbria.ac.uk/id/eprint/2241

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