Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius

Williams, Rachel C., Dawson, Deborah A., Horsburgh, Gavin J. and Sinclair, Billy (2014) Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius. Conservation Genetics Resources, 7 (1). pp. 107-111. Full text not available from this repository.

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Official URL: http://dx.doi.org/10.1007/s12686-014-0304-7

Abstract

A microsatellite-enriched genomic library was created from a single Nautilus pompilius individual and clones/fragments sequenced using Sanger and Illumina MiSeq sequencing. Forty-four markers were tested in four individuals and 30 markers selected for further testing in a population of unrelated individuals from Imperieuse Reef, west Australia. Observed levels of heterozygosity in the IR population ranged from 0.17–1.00 with a mean of 17.2 alleles per locus. No groups of loci displayed evidence of significant linkage disequilibrium. Twenty-one markers were in Hardy–Weinberg equilibrium and will be used to study population structure in this species.

Item Type: Article
Journal or Publication Title: Conservation Genetics Resources
Publisher: Springer Netherlands
ISSN: 1877-7260
Departments: Centre for Wildlife Conservation
Depositing User: Insight Administrator
Date Deposited: 18 Dec 2014 14:19
Last Modified: 18 May 2017 14:07
URI: http://insight.cumbria.ac.uk/id/eprint/1641

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