Williams, Rachel C., Dawson, Deborah A., Horsburgh, Gavin J. and Sinclair, Billy (2014) Isolation and characterisation of microsatellite loci for the ancient cephalopod, Nautilus pompilius. Conservation Genetics Resources, 7 (1). pp. 107-111. Full text not available from this repository.
(Contact the author)Abstract
A microsatellite-enriched genomic library was created from a single Nautilus pompilius individual and clones/fragments sequenced using Sanger and Illumina MiSeq sequencing. Forty-four markers were tested in four individuals and 30 markers selected for further testing in a population of unrelated individuals from Imperieuse Reef, west Australia. Observed levels of heterozygosity in the IR population ranged from 0.17–1.00 with a mean of 17.2 alleles per locus. No groups of loci displayed evidence of significant linkage disequilibrium. Twenty-one markers were in Hardy–Weinberg equilibrium and will be used to study population structure in this species.
Item Type: | Article |
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Journal / Publication Title: | Conservation Genetics Resources |
Publisher: | Springer Netherlands |
ISSN: | 1877-7260 |
Departments: | Research Centres > Centre for National Parks and Protected Areas (CNPPA) |
Depositing User: | Insight Administrator |
Date Deposited: | 18 Dec 2014 14:19 |
Last Modified: | 12 Jan 2024 13:00 |
URI: | https://insight.cumbria.ac.uk/id/eprint/1641 |