Aspden, William, Pegg, Graham, Briskey, Leica and Sinclair, Billy (2006) Species-specific PCR primers for the mitochondrial genome control region hypervariable region 1 of the reef fish Lutjanus sebae. Molecular Ecology Notes, 6 (2). pp. 499-501. Full text not available from this repository.
Abstract
Lutjanus sebae is an important commercial and recreational fish found throughout the Indo-West Pacific region. Using universal primers for the mitochondrial genome control region hypervariable region 1 (HVR1), we isolated a 385-bp fragment of HVR1 then designed specific primers near each end of this sequence in the conserved regions flanking the hypervariable region. These primers were shown to yield good quantity and quality of product and to be species specific. Of 20 L. sebae studied, there were eight segregating sites and eight haplotypes. These primers will provide a useful tool for population genetics studies, identification of larvae and eggs and for wildlife forensics.
| Item Type: | Article |
|---|---|
| Journal / Publication Title: | Molecular Ecology Notes |
| Publisher: | Blackwell |
| ISSN: | Print ISSN: 1471-8278 Online ISSN: 1471-8286 |
| Departments: | Academic Departments > Science, Natural Resources & Outdoor Studies (SNROS) > Forestry and Conservation |
| Depositing User: | Insight Administrator |
| Date Deposited: | 14 Oct 2011 10:37 |
| Last Modified: | 11 Jan 2024 19:16 |
| URI: | https://insight.cumbria.ac.uk/id/eprint/1096 |
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