Species-specific PCR primers for the mitochondrial genome control region hypervariable region 1 of the reef fish Lutjanus sebae.

Aspden, William, Pegg, Graham, Briskey, Leica and Sinclair, Billy (2006) Species-specific PCR primers for the mitochondrial genome control region hypervariable region 1 of the reef fish Lutjanus sebae. Molecular Ecology Notes, 6 (2). pp. 499-501. Full text not available from this repository.

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Abstract

Lutjanus sebae is an important commercial and recreational fish found throughout the Indo-West Pacific region. Using universal primers for the mitochondrial genome control region hypervariable region 1 (HVR1), we isolated a 385-bp fragment of HVR1 then designed specific primers near each end of this sequence in the conserved regions flanking the hypervariable region. These primers were shown to yield good quantity and quality of product and to be species specific. Of 20 L. sebae studied, there were eight segregating sites and eight haplotypes. These primers will provide a useful tool for population genetics studies, identification of larvae and eggs and for wildlife forensics.

Item Type: Article
Journal or Publication Title: Molecular Ecology Notes
Publisher: Blackwell
ISSN: Print ISSN: 1471-8278 Online ISSN: 1471-8286
Departments: Faculty of Health and Science > Science, Natural Resources and Outdoor Studies > Forestry, Conservation & Applied Science
Depositing User: Insight Administrator
Date Deposited: 14 Oct 2011 10:37
Last Modified: 26 Aug 2016 16:09
URI: http://insight.cumbria.ac.uk/id/eprint/1096

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