MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia

Pegg, Graham, Sinclair, Billy, Briskey, Leica and Aspden, William (2006) MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia. Scientia Marina, 70 (Supp 2). pp. 7-12. Full text not available from this repository.

Official URL: http://www.icm.csic.es/scimar/index.php/secId/6/Id...

Abstract

Planktonic larvae were captured above a shallow coral reef study site on the Great Barrier Reef (GBR) around spring-summer new moon periods (October-February) using light trap or net capture devices. Larvae were identified to the genus or species level by comparison with a phylogenetic tree of tropical marine fish species using mtDNA HVR1 sequence data. Further analysis showed that within-species HVR1 sequence variation was typically 1-3%, whereas between-species variation for the same genus ranged up to 50%, supporting the suitability of HVR1 for species identification. Given the current worldwide interest in DNA barcoding and species identification using an alternative mtDNA gene marker (cox1), we also explored the efficacy of different primer sets for amplification of cox1 in reef fish, and its suitability for species identification. Of those tested, the Fish-F1 and -R1 primer set recently reported by Ward et al. (2005) gave the best results.

Item Type: Article
Journal or Publication Title: Scientia Marina
Publisher: Institut de Ciencies del Mar de Barcelona
ISSN: Print ISSN: 0214-8358 Online ISSN: 1886-8134
Related URL(s):
Departments: Faculty of Health and Science > Science, Natural Resources and Outdoor Studies > Forestry, Conservation & Applied Science
Depositing User: Insight Administrator
Date Deposited: 06 Jan 2012 15:07
Last Modified: 26 Aug 2016 16:09
URI: http://insight.cumbria.ac.uk/id/eprint/1095

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